Countercurrent chromatography (CCC) is the form of liquid-liquid chromatography in which, centrifugal or gravitational force is employed to maintain a bed of one liquid phase in a coil or train of chambers, in the absence porous supporting matrix, while a stream of second immiscible phase is pumped through the system in contact with the stationary phase.
- One liquid is held as stationary phase
- The SP retains 40-90% of the total volume of column
- The liq. SP is stable as long as the gravitational/centrifugal force exists
- The advantages of having liquid SP are
- High loading capacity
- Very simple solute retention mechanism
- Either phase can be used as mobile phase
- No pH problem
- Other is pumped through it with good retention of SP
- A series of simultaneous Mixing and Settling zones occur in the column
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