•N - glycosylation – Oligosaccharide structure bind to protein at nitrogene moiety. (Asn-x-Thr/ser)
•O- glycosylation - Oligosaccharide structure bind to protein at oxygen moiety. (Threonyl and seryl )
•Glycoforms: Glycoproteins with same amino acid sequence but different oligosaccharide structure.
•Specific glycosylation pattern differs between cell lines( Cell line is tissue mimicking the in vivo condition for surrounding environment and metabolism.)
–CHO cells - Absence of bisecting N-acetylglucosamine.
–Mouse cell line - Generate terminal galactose units.
–Immunogenic in humans.
according to the Glycosylation Analysis of Protein and your post here, we can conclude that the protein Glycosylation occupy a lot of field in biology.
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