A HPLC system used for separation of components is connected to Mass spectrometer which is the detector as HPLC (Liquid chromatography)
Molecular weight from 30 – 3000 amu can be detected
PARTS OF LC-MS
(1)HPLC Constitutes the LC part
(2)Mode of injection the sample
a. Direct infusion
b. Flow injection analysis FIA
c. Column
(3)SOURCE FOR THE PRODUCTION OF IONS
ION SPARY
HEATED NEBULIZER
(4)INTERFACE: Most important and critical. Ions are transferred from LC to MS region
a.MOVING BELT
b.DIRECT INJECTION
c.PARTICLE BEAM
d.THERMOSPRAY
e.FRIT- FAB (Fast Atomic Bombardment)
f.ELECTROSPRAY IONISATION
g.ATMOSPHERIC PRESSURE IONISATION
h.MALDI ASSISTED LASER DESORPTION WITH TIME OF FLIGHT(MALDI-TOF)
i.TANDEM MS
(5) MS Analyzer: Quadrupole consists of four rods and acts as mass filter
(6) DETECTOR: Channel electron multiplier (CEM)generates a current pulse for every ion that strikes the surface. Number of pulses detected at a given time reflects abundance of the ion in the spectrum.
(7) GPIB box: Communicates between the computer and the main instrument
(8) SOFTWARE:
(9) PRINTER:
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