In bioinformatics, Basic Local Alignment Search Tool, or BLAST, is an algorithm for comparing primary biological sequence information, such as the amino-acid sequences of different proteins or the nucleotides of DNA sequences.
A BLAST search enables a researcher to compare a query sequence with a library or database of sequences, and identify library sequences that resemble the query sequence above a certain threshold.
BLAST p- compare aminoacid query sequences
BLAST n- compare nucleotide query sequences
BLAST x- compare nucleotide query sequence translated in all reading frames against protein sequence database
t BLAST n- compare protein query sequence against nucleotide query sequence database dynamically translated I all reading frames
t BLAST x- compare 6-frame translations of nucleotide query sequence against 6-frame translation of nucleotide sequence database
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