What is Aptamer ?
q Aptamer is a folded single stranded oligonucleotide that binds to a molecular target such as a protein.
q Aptamers are ssDNA or RNA oligonucleotides with very high affinity for their target.
Example: Ala-Arg-Leu-Hist-Ser-Meth
Mechanism of action : It selectively binds to particular protein or target and renders its activity.
•Therapeutically active aptamers inhibit protein-ligand interaction & act as antagonist.
SELEX : Systemic Evolution of Ligand by Exponential enrichment
• Decide the number of nucleotide base, n
• Total types of nucleotide base, 4(A,T,G,C)
• 4n possible different sequences called nucleic acid library
• Immobilize target Protein or Ligand in column.
• Pass the different oligonucleotide in column.
• Separate the oligonucleotide having highest affinity.
| Therapeutic Application | |
α- Thrombin | Prevent thrombosis |
HIV-1 reverse transcriptase | Inhibit Viral replication |
Immunoglobulin E | Prevent Allergy |
Vascular Endothelial Growth Factor | Prevent neovascularization |
Acetylcholine specific auto antibody | Treat Myasthenia gravis |
Platelet derived growth factor | Prevent tumor development |
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